The PfiAT complex exhibited autoregulation by binding to a palindrome (5′- AATTCN 5 GTTAA-3′) overlapping the -35 region of the TA operon. The PfiT toxin directly binds to PfiA and functions as a corepressor of PfiA for the TA operon. We thus suggest the toxin PA0729 be named PfiT for Pf4 inhibition toxin and Rorf0727 be named PfiA for PfiT antitoxin. Importantly, we found that the deletion of the toxin gene PA0729 greatly increased Pf4 phage production. aeruginosa PAO1, we provided experimental evidence to demonstrate that PA0729 and the upstream ORF Rorf0727 near the right attachment site of Pf4 form a type II toxin/antitoxin (TA) pair. By studying the accessory genes in the Pf4 prophage in P. The genomes of Pf prophages contain not only core genes encoding functions involved in phage replication, structure and assembly but also accessory genes. Pf prophages are ssDNA filamentous prophages that are prevalent among various Pseudomonas aeruginosa strains.
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